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Rosmarinic Acid Synthesis in Transformed Callus Culture of Coleus ...
Sixteen diversified transformed callus cultures were maintained for several years in the absence of . Higher sucrose concentrations induce RA synthesis in .

A rice antisense SPK transformant that lacks the accumulation of ...
Despite a reduced sink strength, no difference was found in the sucrose concentration in phloem sap of the transformants and wild-type plants, which increased .

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February 15, 2005


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Establishment of Withania somnifera Hairy Root Cultures for the ...
The. 14 composition of culture medium, especially the sucrose concentration, is known to. 15 influence the growth of transformed roots (Giri and Narasu 2000; .

The slight increase in sucrose concentration was likely due . the enzyme, sucrose and total sugar concentration data were transformed using the logarithmic .

Anthraquinone production by transformed root ... - ScienceDirect
Anthraquinone production by transformed root cultures of Rubia tinctorum: Influence of phytohormones and sucrose concentration. Kyoko Sato Corresponding .

TRANSFORMATION OF SUGARS IN PLANTS. 115 retically, when equilibrium is reached, a finite amount of sucrose must remain in solution, its concentration .

Sucrose Uptake byDeveloping Soybean Cotyledons'
sucrose concentration can be predicted. This can be represented by an Eadie- Hofstee transform of sucrose uptake data over the. 3Abbreviations: Caps .

Transforming Chlamydomonas
treatment increases the transformation rate of cw-15 mutants. 5. Work in lots of a few . want to adjust the sucrose concentration accordingly. Don has suggested .

To induce germination of selected somatic embryos sucrose concentration was increased up to. 40 g. Multiplication of regenerated control and transformed .

Transformation system for Camelina sativa - Patent application
Jul 26, 2012 . [0003] Genetic transformation of plants allows introduction of genes of any origin into the . Sucrose concentrations of 2-6% gave best results.

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February 15, 2005


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Mg2+-free buffer elevates transformation efficiency of Vibrio ...
Jan 16, 2009 . We tried different buffers and conditions, aiming to elevate its transformation efficiency. METHODS AND RESULTS: MgCl(2) and sucrose are .

Genotype X Sucrose Interactions for 2,4— D— induced Somatic ...
that the effects of genotype, sucrose concentration, and genotype >< sucrose interaction were all highly . good target tissue for transformation via particle .

Sucrose Effects On Phenolic Concentration and Plant Regeneration ...
from both explants of all lines at 0% sucrose concentration. However, at this . is recalcitrant with respect to in vitro culture and genetic transformation. (Tetu et al.

Use of phosphomannose isomerase-based selection system for ...
on mannose-selection medium was obtained when mannose/sucrose concentration in the regeneration medium was. 5/15 g dm-3. The best transformation .


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February 16, 2005


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A Rice Antisense SPK Transformant that Lacks the Accumulation of ...
Despite a reduced sink strength, no difference was found in the sucrose concentration in phloem sap of the transformants and wild-type plants, which increased .

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February 16, 2005



posts 100

High root biomass production in anchored Arabidopsis plants grown ...
genetic transformation (5,7). The validity of . The presence of sucrose in the media dramatically increases . and sucrose concentrations were altered for growth .

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February 16, 2005



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Agrobacterium tumefaciens-mediated transformation of Colletotrichum
rather than protoplasts for transformation. This is the only . an actively growing plate, pelleted, washed 2 x, concentration adjusted to 1x106 spores/ml) in a 1.5 .

Sucrose transport in the phloem: integrating root responses to ...
For example, is there a change in phloem sucrose concentration or is there . a transgenic plants transformed with a Pht1;4 promoter–luciferase construct.

Changes in fruit sugar concentrations in response to assimilate ...
fect; it was not subject to intense metabolic transformation. Sorbitol was the most . In peach fruit, sucrose concentration increases with in- creasing leaf:fruit ratio, .

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February 16, 2005


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Effect of Sucrose Concentration and Cultivation Time on Batch ...
Received 28 March 2002. The effect of the initial sucrose concentration (50 g dm . is based on transformation of sucrose by enzymes with a transfructosylating .

Out of three sugars low concentration (0.25%- 2.5%) of maltose and sucrose exhibited the . transformation of energy which are required for growth of cell.

sucrose concentration and transformation

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February 17, 2005


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Electro-transformation of Lactobacillus spp. - OpenWetWare
Sep 16, 2011 . Electro-transformation of Lactobacillus spp. . ALL ICE COLD; Buffer = 0.3M Sucrose; Concentration = 1/120; Voltage = 7,500 V/cm; Recovery .

Sweetness concentration-response behavior of rebiana at room and ...
Oct 20, 2011 . The resultant sigmoid curves were linearized by transforming the . The latter were deemed to be the sucrose concentration at which 50% of .

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February 17, 2005


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Growth characteristics of rice cell genetically modified for ...
Nov 1, 2002 . While growth yield of the non-transformed rice cell was 0.38 for initial sucrose concentration of 30g/l, those values were larger than 0.5 for initial .

Phytohormone-mediated transformation of sugars to starch in ...
Feb 1, 2002 . Phytohormone-mediated transformation of sugars to starch in . At all sucrose concentrations, the effects of ABA and IAA were opposite.

Transformation of Bacillus amyloliquefaciens protoplasts with ...
The highest transformation frequency with plasmid DNA was obtained with a . the same sucrose con- centration as in SMM for phenotypic expression of the .

Brazilian Oral Research - The effect of iron on Streptococcus mutans ...
These biofilms were exposed eight times/day to 10% sucrose, and two times/day . of the viable bacteria and concentration of extracellular polysaccharides (EPS). . enamel showed non-normal distribution, so they were log transformed.18 .

Genetic variability in a population of arbuscular mycorrhizal fungi ...
significant interaction terms. Growth of transformed carrot roots decreased consider- ably with the reduced sucrose concentrations (Fig. 2a,. F2,142 = 247.35, .

Transformation of Brassica napus and Brassica oleracea Using ...
ABSTRACT. An efficient and largely genotype-independent transformation . sugar source and concentration was found to be 2% sucrose, on which many .

Full Text - Microbiology
At higher concentrations of sucrose, levansucrase catalyses the formation of high . All the strains constructed were obtained by transformation with replicative or .

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February 17, 2005


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Transformation of Alachlor by Microbial Communities
Transformation of alachlor by pure cultures of the soil fungi. Fusarium . 14C labeled alacllor and sucrose concentrations of 0, 0.01, and 0.1%, respectively, TSI .

Transformation of Magnaporthe grisea to phosphinothricin ...
Three transformation systems have been reported for the rice blast fungus Magnaporthe . in complete medium amended with a high concentration of PPT ( 400 µg/ml). . Instead of 1.2 M sorbitol, 20% sucrose was used as osmotic stabilizer.

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February 17, 2005



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Team:Brown-Stanford/PowerCell/NutrientSecretion -
The solution to this was transformation by more traditional means of the cargo plasmid . Work is currently underway to assay the sucrose concentrations that are .

Design of Freeze-Drying Processes for Pharmaceuticals: Practical ...
long enough such that all solution has transformed into solid. Because of the . At a sucrose concentration of 80% w/w, the systems are at the maxi- mum freeze .

Ciência e Agrotecnologia - Factors affecting the production and ...
Sucrose concentrations of 1.2 mol l-1 and 1 mol l-1 were the most suitable osmotic . Molecular transformation has facilitated the understanding of biological .

Sugar Uptake and Translocation in the Castor Bean Seedling. II - JStor
Sugar Transformations During Uptake1 . During sucrose uptake by the cotyledons of castor bean seedlings excised from their endosperms, . rate of fruetose uptake exceeded that of glucose at concentrations up to 0.5 M. Sucrose uptake .

Transformation Protocols
. to the fresh selection media (with the same hormone/antibiotic concentration, . The regeneration medium consisted of full-strength MS medium, 3% sucrose, . and 50 mg/l kanamycin to control bacterial growth, and select for transformed .

Handbook of Photosynthesis, Second Edition
D. Sucrose Biosynthesis. III. Long-Term In Vivo Effects of Pi Deprivation. A. Plant Growth Response and Phosphate Concentration. B. Photosynthetic Machinery .

Optimization of electrotransformation conditions for Leuconostoc ...
Sep 4, 2012 . Aims: To establish an efficient genetic transformation protocol for . 2% sucrose and incubated for 1 h at 30°C. The transformed cells were spread on . Low concentration treatment with lysozyme improved the transformation .

Microsoft Word - Complete_Proceedings_version3.doc
Transformation and Dihaploid Production for Flax and Linseed Breeding . Higher concentrations of sucrose supported rhizogenesis in shoot-tip explants and in .

Soybean Somatic Embryogenesis and Transformation
Soybean Somatic Embryogenesis and Transformation . FN medium, with sucrose concentration lowered to 3%, Standard FN medium, FN Lite medium .

Plant Energy Transport
This raises the concentration of sucrose molecules in the companion cells above that in the sieve tubes, so they can then move into the sieve tubes by diffusion.

Concentration?dependent sugar preferences in nectar?feeding birds ...
We predicted that at high concentrations they would prefer sucrose over hexoses, . on arcsine square root transformed preference values (Sokal & Rohlf 1995).

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December 16, 2005


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Growth Vessel and Substrate Affect Anthurium Micropropagation
not only a theoretical prerequisite for plant transformation, but it is employed in . Different concentrations i.e. 1, 1.5, 2, 2.5 and 3% of sucrose were added to MS .

Effect of pH, counter ion, and phosphate concentration on the glass ...
METHODS: Freeze-dried trehalose and sucrose systems containing different . the Tg and by Fourier-transform infrared spectroscopy (FTIR) analysis to evaluate .

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December 16, 2005


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Potential of Sucrose and Pennisetum purpureum cv. Cameroon ...
sugar concentration. Figure 1 - Proportion of immobilized juveniles (data transformed to arcsine?p), exposed to sucrose concentrations (dissolution of brown .

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Plant Methods | Full text | A novel fluorescent assay for sucrose ...
We tested a variety of incubation times, esculin concentrations, and buffer pH values . Yeast (SEY6210) transformed with plant sucrose transporters StSUT1, .

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